Additional files [RJEE Vol. 8 (4). 2023 – 1]

• Fig. 1. Dendrogram of phylogenetic relationships of roe deer (Capreolus sp) haplotypes from the Penza region based on sequence analysis of the control region (CR, n = 61, 934 bp, model T92+G+I, BIC = 6512.5, InL = – 2583.3) mtDNA using the maximum likelihood method (ML). The outgroup is the red deer (Cervus elaphus maral). The designation Cap is used for samples from the Penza region. The numbers at the nodes indicate the level of bootstrap support
• Fig. 2. Dendrogram of phylogenetic relationships of roe deer (Capreolus sp) haplotypes from the Penza region based on analysis of Cyt b gene sequences (n = 24, 1139 bp, model TN93, BIC = 5337.8, InL = – 2412.2) mtDNA using the maximum likelihood (ML) method. The outgroup is the red deer (Cervus elaphus maral). The designation Cap is used for samples from the Penza region. The numbers at the nodes indicate the level of bootstrap support
• Fig. 3. Genetic distances (p-distance) between formalized groups of roe deer according to two mitochondrial DNA markers (CR, Cyt b). CC – European roe deer, CP – Siberian roe deer, CPR – roe deer from the Penza region. Minimum distances are highlighted in red
• Fig. 4a. Detailed dendrogram (a) of phylogenetic relationships of roe deer haplotypes (Capreolussp) from the Penza region (groups A, B, C, D, E, n = 18) based on sequence analysis of the control region (CR, n = 61, 934 bp, model T92+G+I, BIC = 6512.5, InL = – 2583.3) mtDNA by maximum likelihood (ML) method. The outgroup is the red deer (Cervus elaphus maral). The designation Cap is used for samples from the Penza region. Haplogroups on the dendrogram and on the map (б): A – Kameshkirsky, Lopatinsky, Nikolsky, Bessonovsky, Kuznetsk districts; B – Kameshkirsky; C – Zemetchinsky, Luninsky; D – Maloserdobinsky, Penzensky; E – Kuznetsky, Sosonovborsky. The numbers at the nodes indicate the level of bootstrap support. The numbers of points on the map correspond to the numbers of samples (Table 1)
• Fig. 4b. Detailed dendrogram (a) of phylogenetic relationships of roe deer haplotypes (Capreolussp) from the Penza region (groups A, B, C, D, E, n = 18) based on sequence analysis of the control region (CR, n = 61, 934 bp, model T92+G+I, BIC = 6512.5, InL = – 2583.3) mtDNA by maximum likelihood (ML) method. The outgroup is the red deer (Cervus elaphus maral). The designation Cap is used for samples from the Penza region. Haplogroups on the dendrogram and on the map (б): A – Kameshkirsky, Lopatinsky, Nikolsky, Bessonovsky, Kuznetsk districts; B – Kameshkirsky; C – Zemetchinsky, Luninsky; D – Maloserdobinsky, Penzensky; E – Kuznetsky, Sosonovborsky. The numbers at the nodes indicate the level of bootstrap support. The numbers of points on the map correspond to the numbers of samples (Table 1)
• Fig. 5. Distribution of haplotypes (n = 8) of the mtDNA control region (CR) of roe deer in the Penza region. Regionally specific haplotypes are indicated by gray pins with red inscriptions
• Fig. 6. Distribution of haplotypes of the mtDNA Cyt b gene (n = 8) of roe deer in the Penza region. Regionally specific haplotypes are indicated by gray pins with red inscriptions
• Fig. 7. Results of genetic analysis of nucleotide sequences (n = 60) of the mtDNA region (CR, 1036 bp) of Siberian and European roe deer – median network of haplotypes (Hap, n = 45). The length of the branches connecting individual haplotypes is proportional to the number of mutational steps (indicated by notches). CP – Siberian roe deer, haplotypes Hap_1, Hap_3, Hap_31–45 – Cp41, Cp42, Cp38–Cp40 and Cp43–Cp54, respectively (NCBI); CC – European roe deer, haplotypes Hap_7–30 – Spain, L1–L24, respectively (NCBI); CC – European roe deer, haplotypes Hap_7–30 – Spain, L1–L24, respectively (NCBI); CPR – roe deer from the Penza region, haplotypes Nar_1 (Cap1, Cap3, Cap5, Cap6, Cap7, Cap9, Cap10, Cap12, Cap14, Cap15, Cap16), Hap_2 (Cap2), Hap_3 (Cap4), Hap_4 (Cap8, Cap17), Hap_5 (Cap11, Cap13), Hap_6 (Cap18), numbers are the same as in the table. 1