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  • Fig. 1. Distribution data of the marsh frogs in the Kazakh Upland collected during the last 70 years (A) with the record details of the inset with oblique shading (B). The data obtained before 2005 were taken from [8]. The data for the period from 2006–2020 are from an unpublished database of the first author; and the data for 2021 were obtained by the authors of the present paper (APPENDIX I)
  • Fig. 2. Electropherogram of the products of restriction of mtDNA of ND2 gene of the two marsh frog forms by endonuclease BsuRI (HaeIII). Wells of the gel: no. 1 are «Balkhash»; nos. 2, 3 are P. cf. bedriagae. Numbers on the right are the lengths of the mtDNA fragments, base pairs. M is the marker of the molecular length
  • Fig. 3. The habitats of the marsh frogs in the Kazakh Upland: А – Irtysh-Karaganda Channel, July 22, 2021; B – Shubartubek Peninsula, north-west bank of the Balkhash Lake, May 15, 2021; C – the spring at the piedmont of Bektau-Ata Mount (north of Balkhash Lake) with shallow and poor vegetation which is not typical for the marsh frogs, May 12, 2021; D – the bank of the Shar River (left tributary of the Irtysh River), south of Semipalatinsk, August 24, 2021. Photo by: А. Kaptyonkina (A), Т. Dujsebayeva (B, С), V. Khromov (D)
  • Fig. 4. The areas highlighted by yellow color (incut) and by dotted line (main picture) are the boundaries of the Central Kazakhstan Upland. The inserted map represents ND2 haplogroups based on molecular-genetic analysis, whereas the red circles represent the Anatolian form, P. cf. bedriagae, and the purple color the Balkhash native form. The size of of the circle demonstrates the sampling size, whereas the numbers indicate the sampling sites
  • Fig. 5. Annual precipitation (A) and average air temperature (B) for the Karaganda meteorological station according to polynomial analysis data (according to [37] with changes). The red lines indicate the periods of expansion (left) and retreat (right) of the marsh frogs in the Kazakh Upland

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