Additional files [RJEE Vol. 6 (3). 2021 – 2]

• Fig. 1. Distribution of mtDNA haplotypes (D-loop) in geographically separated breeding groups of the imperial eagle (Aquila heliaca) in the Volga region. D, E … RF, RG – identified haplotypes (n = 14); geographically separated groups of eagles: 1 – northwest of the Ulyanovsk region (UlR-NW); 2 – northeast of the Ulyanovsk region (UlR-NE); 3 – center of the Ulyanovsk region (UlR-C); 4 – south of the Ulyanovsk region (UlR-S); 5 – Republic of Tatarstan (RTat)
• Fig. 2. Dendrogram of phylogenetic relationships of haplotypes of imperial eagles Aquila heliaca on the basis of analysis of mtDNA control region sequences (345 bp) using the Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods (Kimura 2-parameter models). Outgroup – spanish imperial eagles (A. adalberti). The numbers in the nodes indicate the level of bootstrap support (10 000 bootstrap replications). AqH_1, AqH_2, AqA – identified haplogroups. An asterisk (*) denotes haplotypes found on the territory of the Ulyanovsk region as of 2016 [8], two asterisks (**) – haplotypes found on the territory of the Ulyanovsk region as of 2021, three asterisks (***) – haplotype found on the territory of Tatarstan [9]. Photo of birds in the figure: A. heliaca – © M. Korepov, A. adalberti – © Ricardo Rodriguez
• Fig. 3. Median haplotype network based on mtDNA control region sequences (345 bp) of imperial eagles Aquila heliaca (n = 75). Transverse strokes and numbers on the branches show the number of mutational events. The colors indicate haplotype samples with different geographical affiliation: GenHap – haplotypes not discovered in the Volga region (NCBI GeneBank), UR-NW – northwest of the Ulyanovsk region, UR-NE – northeast of the Ulyanovsk region, UR-C – center of the Ulyanovsk region, UR-S – south of the Ulyanovsk region, TAT – Republic of Tatarstan, Ch – Chuvash Republic, Mord – Republic of Mordovia. The circle size is proportional to the number of individuals, and the fractions are proportional to the frequencies of haplotypes
• Fig. 5. Distribution of mtDNA haplotypes (D-loop) in geographically separated breeding groups of the imperial eagle (Aquila heliaca) in Eurasia. D, E … RF, RG – identified haplotypes (n = 16); geographically separated groups of eagles: 1 – Western Slovenia (WestSlov); 2 – Eastern Slovenia (EastSlov); 3 – Eastern Ukraine (EastUkr); 4 – Ulyanovsk Region (UlR); 5 – Republic of Tatarstan (RTat); 6 – Orenburg Region (OrenbR); 7 – Republic of Kazakhstan, west and center (RKazakh); 8 – Naurzumsky Reserve, Northern Kazakhstan (NurKazakh); 9 – Republic of Khakassia (RKhak); 10 – Republic of Altai (RAlt)
• Fig. 4. Model of divergence of nest groups of the imperial eagles (Aquila heliaca) on the territory of the Ulyanovsk region relative to clusters (K = 5). Breeding groups: 1 – northwest of the Ulyanovsk region (UR-NW); 2 – northeast of the Ulyanovsk region (UR-NE); 3 – center of the Ulyanovsk region (UR-C); 4 – south of the Ulyanovsk region (UR-S)